Coding
Part:BBa_K1141006:Design
Designed by: Sarah Amblard Group: iGEM13_Grenoble-EMSE-LSU (2013-10-04)
pBad-RBS-sspB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RBS was added by PCR as we struggled isolating it by miniprep.
Source
This part contains pBad BBa_K206000, RBS elowitz BBa_B0034 and sspb BBa_K174000.