Coding

Part:BBa_K1141006:Design

Designed by: Sarah Amblard   Group: iGEM13_Grenoble-EMSE-LSU   (2013-10-04)


pBad-RBS-sspB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS was added by PCR as we struggled isolating it by miniprep.


Source

This part contains pBad BBa_K206000, RBS elowitz BBa_B0034 and sspb BBa_K174000.

References